Thermo Scientific Phusion Site-Directed Mutagenesis Kit is a versatile and efficient tool for introducing point mutations, insertions, or deletions in any type of plasmid DNA
With this kit, the entire plasmid is amplified using phosphorylated primers that introduce the desired changes
The amplified, linear PCR product, containing the desired mutation, is circularized in a 5-minute ligation reaction with T4 DNA Ligase
The resulting plasmid can be then transformed into any competent
The optimal annealing temperature for Phusion DNA Polymerases may differ significantly from that of Taq-based polymerases
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